New adaptive laboratory evolution database highlights the need for consolidating directed evolution data

Laboratory-directed protein evolution.

Systematic approaches to directed evolution of proteins have been documented since the 1970s. The ability to recruit new protein functions arises from the considerable substrate ambiguity of many proteins. The substrate ambiguity of a protein can be interpreted as the evolutionary potential that allows a protein to acquire new specificities through mutation or to regain function via mutations t...

Stress-directed adaptive mutations and evolution.

Comparative biochemistry demonstrates that the metabolites, complex biochemical networks, enzymes and regulatory mechanisms essential to all living cells are conserved in amazing detail throughout evolution. Thus, in order to evolve, an organism must overcome new adverse conditions without creating different but equally dangerous alterations in its ongoing successful metabolic relationship with...

Adaptive Query Formulation to Handle Database Evolution

Databases are continuously evolving environments, where design constructs are added, removed or updated rather often. Research has extensively dealt with the problem of database evolution. Nevertheless, problems arise with existing queries and applications, mainly due to the fact that, in most cases, their role as integral parts of the environment is not given the proper attention. Furthermore,...

Developing Adaptive Differential Evolution as a New Evolutionary Algorithm, Application in Optimization of Chemical Processes

Directed evolution: new parts and optimized function.

Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understanding, directed evolution is a powerful complement to 'rational' engineering a...